DNA

Part:BBa_J119007:Design

Designed by: Todd Eckdahl   Group: Eckdahl Lab   (2011-07-22)


HPP Assembly Vector pSB1A2 with Promoter-BsmBI-BsaI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 68
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 13
    Illegal NheI site found at 36
    Illegal PstI site found at 68
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 68
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 68
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 57


Design Notes

Standard BioBrick prefix and suffix are not used. Only a EcoRI site is used as a prefix and a Pst I site as a suffix.


Source

Cloned from synthetic oligonucleotides into pSB1A2 EcoRI + PstI vector.

References